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Acta medica Lituanica

ISSN 1392-0138
ISSN 2029-4174 (online)

 2009 m. Nr. 3-4

The use of high-resolution melting analysis for Salmonella spp. CRISPR sequence genotyping
Maksim BRATČIKOV, Mykolas MAURICAS

Background. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) of Salmonella spp. and of other bacteria continuously evolve due to the rearrangements of fragments. This phenomenon forms a high degree of strain polymorphism. Real-time PCR and high-resolution DNA melting (HRM) analysis of CRISPR regions provide a possibility for a rapid evaluation of strain polymorphism. Our intent was to apply the HRM technique for Salmonella spp. genotyping.
Materials and methods. Salmonella spp. strains DNA were amplified and analyzed with HRM using Rotor-Gene 65H0-100.
Results. Forty-nine Salmonella spp. strains were analyzed, and 23 genotypes were identified for each CRISPR (CRISPR1 and CRISPR2). We have found the genotypes of both CRISPRs to be related to the same Salmonella spp. serotypes.
Conclusions. The CRISPRs HRM method is a valuable tool for Salmonella spp. genotyping.

Keywords: CRISPR, HRM, Salmonella spp. genotyping, Rotor-Gene 6000, Syto9
Issues:

2011 - Vol.18
No. 1, No. 2, No. 3, No. 4

2010 - Vol.17
No. 1-2, No. 3-4

2009 - Vol.16
No. 1-2, No. 3-4

2008 - Vol.15
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2007 - Vol.14
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2006 - Vol.13
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2005 - Vol.12
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2004 - Vol.11
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2003 - Vol.10
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2002 - Vol.9
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2001 - Vol.8
No. 1, No. 2, No. 3, No. 4