ISSN 1392-0138
ISSN 2029-4174 (online)
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2009 m. Nr. 3-4
 Quantitative changes of functionally different CD8hCD57+ T-cell subsets in the peripheral blood of advanced renal cell carcinoma or high-risk melanoma patients
Marius STRIOGA, Neringa DOBROVOLSKIENĖ, Aloyza LUKŠIENĖ, Tadas PETRAITIS, Jolita GIBAVIČIENĖ,
Rasa PURVINIENĖ, Nijolė KAZLAUSKAITĖ, Dainius CHARACIEJUS, Vita PAŠUKONIENĖ
Introduction. CD8hCD57+ T-cell subpopulation and its functionally different subsets play
an important role in antitumour immunity. The relation of competitive subsets may influence
the overall effect of CD8hCD57+ T-cell mediated antitumour immunity and determine
an individual response to antitumour immunotherapy. The aim of this study was to evaluate
the proportion of cytotoxic, immunomodulating and immunosuppressive subsets of
the CD8hCD57+ T-cell subpopulation in the peripheral blood of cancer patients and agematched
healthy controls.
Materials and methods. We studied the expression of biomarkers representing the cytotoxic
(perforin), immunosuppressive (FOXP3, NKG2A) and immunomodulating (IFNγ)
properties of CD8+CD57+ T cells in the peripheral blood of 49 cancer patients: 30 with
clear cell renal cell carcinoma (age median 58, range 43–81), 19 with high risk cutaneous
melanoma (age median 68, range 45–86) and 26 controls (age median 55, range 41–81) by
multicolour flow cytometry.
Results. The percentage of immunosuppressive CD8hCD57+FOXP3+ T-cell subset in
CD8hCD57+ T-cell population varied. It was absent in 65% of controls, while only 23% and
26% of such patients were observed in renal cell carcinoma (RCC) and melanoma groups,
respectively. Even 40% of RCC and 37% of melanoma patients had a high percentage of
CD8hCD57+FOXP3+ T-cell subset, while in the control group we found no such subjects.
The cytotoxic CD8hCD57+Perforin+ T-cell subset was significantly increased in RCC
patients, but showed no relevant rise in melanoma patients, whereas the immunomodulating
CD8hCD57+IFNγ+ subset was significantly increased in melanoma patients but showed
no relevant rise in RCC patients when compared to controls.
Conclusions. The amount of various functionally different subsets in CD8hCD57+
T-cell subpopulation varies greatly among cancer patients. These differences may influence
the overall CD8hCD57+ T-cell mediated antitumour immune response and determine an
individual response to antitumour immunotherapy.
Keywords: immunosuppressive CD8hCD57+FOXP3+ T cells, cytotoxic CD8hCD57+Perforin+
T cells, immunomodulating CD8hCD57+IFNγ+ T cells, renal cell carcinoma, cutaneous
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