Biologija / Biology
 ISSN 1392-0146 ISSN 2029-0578 (online) |
2004 m. Nr. 1 Synergistical effect of NO and Ca2+ on mitochondrial function
Nitric oxide (NO) and Ca2+ concentration in tissue is reported to increase during various myocardial pathologies, including ischemia. In this study, we investigated the effect of 4 μM NO on isolated rat heart mitochondria in the presence of 5 μM of free Ca2+, conditions resembling the ischemic situation. We found NO together with Ca2+ to inhibit the NAD-substrate oxidation pathway due to inactivation of Complex I. Inhibition of Complex I was prevented by superoxidedismutase and urate, and reversed by light and thiols, suggesting the inhibition that might occur due to nitrosation of some critical thiols by peroxynitrite.
Keywords: mitochondria, nitric oxide, respiration, nitrosation, Complex I, peroxynitrite |
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