Biologija / Biology
 ISSN 1392-0146 ISSN 2029-0578 (online) |
2004 m. Nr. 1 Some quinone derivatives as redox mediators for PQQ-dependent glucose dehydrogenase
Some quinone derivatives (2,3-dichloro-1,4-naphthoquinone, 2-methyl-6-methoxy-1,4-benzoquinone, 1,4-naphthoquinone, 2-hydroxy-1,4-naphthoquinone, trimethyl-1,4-benzoquinone, tetramethyl-1,4-benzoquinone, 9,10-phenanthrenequinone, 2-methyl-1,4-naphthoquinone) were investigated as a redox mediators for pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) purified from Erwinia sp. 34-1. Amperometrical response to glucose addition in the sample containing GDH and quinone was investigated. The results show that 2-methyl-6-methoxy-1,4-benzoquinone, 1,4-naphthoquinone, trimethyl-1,4-benzoquinone are the best redox mediators for GDH.
Keywords: quinones, glucose determination, quinoprotein, glucose dehydrogenase |
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