ISSN 1392-0146
ISSN 2029-0578 (online)
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2004 m. Nr. 1
Dot-immunoperoxidase assay for the demonstration of soluble Fcg receptors
V. MILIUKIENĖ, A. PILINKIENĖ, G. BIZIULEVIČIENĖ
We have developed a sensitive dot-immunoperoxidase assay to demonstrante and characterize the functional activity of soluble Fcγ receptors (sFcγR). Samples containing sFcγR were immobilized on nitrocellulose membrane. Immune complexes of horseradish peroxidase (HRP) and rabbit IgG antibodies to HRP were visualized with an HRP developer. Dot-immunoperoxidase assay is described as a rapid and simple method for the demonstration and characterization of functionally active sFcγR.
Keywords: soluble Fcγ receptor, immune complex, dot-immunoperoxidase
assay
INTRODUCTION
Several dot-immunobinding assays have been described
[1, 2], and the techniques have been useful in
the characterization of autoantigens, allergens and
infectious organis
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