ISSN 1392-0146
ISSN 2029-0578 (online)
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2005 m. Nr. 1
Identification of Ras GTPase-activating proteinbinding
sites in adaptor protein Nck-α
M. Ger,
V. Tunaitis,
M. Stoškus,
M. Valius
Adaptor proteins consisting of Src homology (SH) 2 and 3 domains mediate various cellular signaling events initiated by receptor protein tyrosine kinases. Nck-α is one of the adaptor proteins implicated in the coordination of multiple intracellular signal transduction pathways emanating from the ligand-activated PDGF receptor-b. In our previous studies we have shown that Nck-α constantly associates with RasGTPase-activating protein (RasGAP). Here we show that SH3 domains of Nck-α are responsible for constitutive association with RasGAP. Moreover, Nck-α and RasGAP interact directly in vitro. These data provide a new insight into the molecular mechanism of RasGAP and Nck-α interaction.
Keywords: PDGF receptor, Nck, RasGAP
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