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Biologija / Biology

ISSN 1392-0146
ISSN 2029-0578 (online)

2005 m. Nr. 1

Cadmium ions inhibit activity of translation components and induce cell death in mouse liver
L. Ivanovienė, I. Sadauskienė, L. Ivanov, V. Lesauskaitė, R. Gailevičiūtė, H. Rodovičius, D. Karčiauskaitė

The aim of this study was to determine a relationship between activity of tRNALeu and leucyl-tRNA synthetase and cell death in cadmium-intoxicated mouse liver. 24 h after intoxication of mice with a sublethal dose of cadmium ions (1.6 mg per 1 kg of body mass) the activity of leucyl-tRNA synthetase decreased by 30%, whereas the acceptor activity of mice liver tRNALeu decreased by 54% as compared to the control. Experiments in vitro showed that the activity of mouse liver tRNALeu and leucyl-tRNA synthetase were reduced by 98% and 70%, respectively, when a 20 µM concentration of cadmium ions was present in the reaction mixtures. It is proposed that tRNA molecule is more sensitive than aminoacyl-tRNA synthetase to cadmium action. According to data of a TUNEL assay, the number of apoptotic (TUNEL+) cells was the same in both Cd-intoxicated livers and control. Agarose-gel DNA electrophoresis revealed a diffusional pattern typical of randomly degraded DNA fragments. It is possible to assume that not apoptosis but rather necrosis is a principal mode for elimination of irreversibly damaged liver cells 24 h following cadmium intoxication.

Keywords: cadmium, tRNA, leucyl-tRNA synthetase, apoptosis, necrosis

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