The aim of our study was to assess the use of waterfowl specines specific primers in order to detect polymorphism in Lithuanian native geese breeds (Vištinės, Skarulės and Vištinės-Skarulės hybrids – Native Mixed). Also, the White-fronted geese species was investigated for comparison. The microsatellite DNA analysis was carried out using 11 microsatellite primers from which only 4 gave a positive PCR product: Sfimu1 (some wild waterfowl species specific marker), TTUCG-1, TTUCG-2, TTUCG-4 (Canada geese specific marker). According to our data, it is possible to use wild waterfowl specific microsatellite DNA markers for a comparative microsatellite DNA analysis of the White-fronted geese species and 2 Lithuanian breeds (Vištinės and Skarulės), and their hybrids (Native Mixed) were obtained by interbreeding these breeds. We found that the TTUCG-1 primer, due to monomorphic PCR products, was not suitable for the population analysis of Skarulės and the Vištinės geese breeds. Due to the absence of the amplified product, the TTUCG-4 primer is not suitable for hybrid Gese microsatellite DNA analysis.

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2011 - Vol.57
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2010 - Vol.56
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