The aim of this study was to apply QCM for the registration of synthetic ssDNA fragments binding to SAM and for DNA hybridization. 5’-aminoalkyl- oligonucleotides were successfully covalently immobilized onto SAM /Au via carboxylate ester linkages, and we observed a reduction in the frequency of 91 Hz. For duplex formation, the oligo-50mer after incubation with 10× ligation buffer was placed on an ssDNA-modified surface, and we observed a reduction in the frequency of 112 Hz. It means that covalently we immobilized 629 ng/cm² of 5’-amino-alkyl-oligonucleotides. After the duplex formation, the gold-coated quartz mass increased by 776 ng/cm².

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