Biologija / Biology
 ISSN 1392-0146 ISSN 2029-0578 (online) |
2006 m. Nr. 3 Separation of recombinant porcine growth hormone
monomer from dimer and other oligomers in the
production process from E. coli inclusion bodies
Porcine growth hormone expressed in E. coli accumulates as inclusion bodies. After the refolding process, besides the monomer, also a dimer and higher oligomers of this protein were found. A successful application of hydrophobic chromatography for porcine growth hormone monomer separation from other forms in the production process is described. The results of target protein purification from 4 g of biomass are presented.
Keywords: porcine growth hormone, hydrophobic chromatography, inclusion bodies, aggregation |
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