ISSN 1392-0146
ISSN 2029-0578 (online)
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2006 m. Nr. 4
Synthesis of hepatitis B virus surface protein derivates
in yeast S. cerevisiae
Aistė Bulavaitė,
Rasa Sabaliauskaitė,
Juozas Staniulis,
Kęstutis Sasnauskas
HBV surface proteins PreS1[13–59]-S, PreS1[20–59]-S, PreS1[30–59]-S, PreS1[40–59]-S, PreS1[50–59]-S, PreS1[90–119]-S were produced in S.cerevisiae and purified. Electron microscopy suggested spherical virus-like particle formation for all the proteins except PreS1[90–119]-S. The PreS1[90–119] sequence was demonstrated to decrease protein solubility. Proteins are suitable for Tupaia primary hepatocyte binding investigations, diagnostic products and valcine candidate development.
Keywords: hepatitis B virus, HBV surface proteins, preS1, virus-like particles
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