The propagation of lilac (Syringa vulgaris L.) sorts in vitro was studied in order to improve the propagation protocol. It has been established that a convenient time for single node explant isolation from young shoots is the period when generative buds blossom out. Microcutting type (single node or tip) and cutting density in the cultivation vessel had a little effect on microshoot development during propagation. The media containing 150% Murashige–Skoog (MS) macrosalts stimulated shoot proliferation better than did MS 100% or Anderson’s (1984) macrosalt composition. Cytokinins BAP or 2iP (1; 3 mg/l) together with NAA 0.05 mg/l and IAA 0.15 mg/l improved shoot growth during micropropagation. The ex vitro acclimatization and rooting took a month; the efficiency was approximately 99%. The growth of the plantlets transferred to open field was stimulated fertilizing them with NH₄NO₃ and KH₂PO₄. The fertilization had no effect on the concentration of photosynthetic pigments.

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