 ISSN 1392-0146 ISSN 2029-0578 (online) |
2008 m. Nr. 2 Identification and some properties of Alfalfa mosaic alfamovirus isolated from naturally infected tomato crop
Alfalfa mosaic alfamovirus (AMV) causing disease in tomato (Lycopersicon esculentum Mill.) crop in Lithuania has been isolated and identified on the basis of determination of an experimental host range, symptom expression, morphological properties and a positive immunological test of the virus isolates. The primers designed on the basis of published sequences were applied for amplification of AMV RNA fragments in reverse transcription-polymerase chain reaction (RT-PCR) using host plants experimentally infected with AMV. The detection of AMV in tomato and inoculated test-plants was confirmed by the RT-PCR technique. Analysis of PCR products in polyacrylamide gel electrophoresis revealed amplification of approximately 620 bp (base pair) fragments which were in agreement with the size of the fragment expected from the sequence data. Keywords: tomato, Alfalfa mosaic alfamovirus, identification, ELISA, RT-PCR |
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