 ISSN 0235-7216 ISSN 2424-4538 (online) |
2010 m. Nr. 2-4 The influence of solvent on the quantity and antioxidant activity of ethanolic extracts of Lithuanian propolis
Ethanolic extracts (60%, 70%, 80% and 96%) of propolis were analysed by the spectrophotometric and chromatographic methods for the quantification of bioactive compounds such as caffeic and ferulic acids, pinocembrin, pinostrobin, chrysin and the total content of polyphenols. The influence of ethanol concentration on the content of bioactive compounds and the antioxidant activity by DPPH• inactivation was demonstrated. 60% ethanol extracted the highest amount of caffeic and ferulic acids, whereas 80% ethanol gave the highest yields of pinocembrin, pinostrobin and chrysin. The most potent solvent concentration was 80% for the evaluation of total polyphenols (9.57 ± 0.21 mg/mL GAE and 96% 32.7 ± 0.57 mg/mL PGE). All propolis extracts showed a high DPPH• radical inactivation which ranged from 82.96 ± 1.12% to 75.81 ± 0.14%. Their antioxidant activity decreased with increasing ethanol concentration. There was a significant correlation between DPPH• radical inactivation and the amounts of caffeic acid (r = 0.94) and ferulic acid (r = 0.96) in propolis extracts. Keywords: propolis, ethanolic extract, antioxidant activity |
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