Chemija / Chemistry
 ISSN 0235-7216 ISSN 2424-4538 (online) |
2011 m. Nr. 2 Spectroscopic study of yeast Saccharomyces cerevisiae cell wall structure
The aim of this study was to evaluate the possibility of Fourier transform infrared (FT-IR) difference spectroscopy for the analysis of the cell wall structure of yeast Saccharomyces cerevisiae cells. For this purpose, the wild-type strain p63-DC5 and mutant XCY42-30- D(mnn1) strains, which possessed changes in cell wall polymer synthesis of the cell wall, were used. the spectra of wild and mutant strains differ in the region corresponding to polysaccharides. The intensity of vibration bands at 1 044 cm–1 demonstrated that the content of mannans was slightly increased in the mutant strain. At the same time, differences in the spectral range of vibrations 1 086–1 104 cm–1 showed that the mutant strain also had a higher content of β-(1–3) glucans. The results of the study showed that changes in the spectra were associated with yeast cell wall structure and demonstrated a high sensitivity and simplicity of FT-IR spectroscopy in the evaluation of yeast strains with a different cell wall architecture.
Keywords: FT-IR, Saccharomyces cerevisiae cell wall structure, mnn1 mutant |
Issues:
2017 - Vol.28 No. 1, No. 2, No. 3, No. 4 2016 - Vol.27 No. 1, No. 2, No. 3, No. 4 2015 - Vol.26 No. 1, No. 2, No. 3, No. 4 2014 - Vol.25 No. 1, No. 2, No. 3, No. 4 2013 - Vol.24 No. 1, No. 2, No. 3, No. 4 2012 - Vol.23 No. 1, No. 2, No. 3, No. 4 2011 - Vol.22 No. 1, No. 2, No. 3, No. 4 2010 - Vol.21 No. 1, No. 2-4 2009 - Vol.20 No. 1, No. 2, No. 3, No. 4 2008 - Vol.19 No. 1, No. 2, No. 3-4 2007 - Vol.18 No. 1, No. 2, No. 3, No. 4 2006 - Vol.17 No. 1, No. 2-3, No. 4 2005 - Vol.16 No. 1, No. 2, No. 3-4 2004 - Vol.15 No. 1, No. 2, No. 3, No. 4 2003 - Vol.14 No. 1, No. 2, No. 3, No. 4 2002 - Vol.13 No. 1, No. 2, No. 3, No. 4 2001 - Vol.12 No. 1, No. 2, No. 3, No. 4 |