Isoflavones are phenolic phytochemicals of major interest for health. Clovers are one of their alternative sources. The current study was designed to quantify three isoflavones in various clover species and their aerial plant parts by a high-performance liquid chromatography and to identify the most promising source for medicinal use. Isoflavone contents were quantified in the plants of 11 perennial and 4 annual species of genus Trifolium. Subject to aerial part and perennial clover species, the concentrations of formononetin, daidzein and genistein varied in the wide ranges: <Limit of detection (LOD) – 2.19, <LOD – 1.42 and <LOD – 2.898 mg g^–1, respectively. According to the sum of three isoflavones quantified in leaves–stems–flowers, perennial clover species were arranged as follows: T. medium (6.01–3.50–2.93 mg g^–1) > T. pratense > T. fragiferum > T. alpestre > T. rubens > T. ochroleucum > T. pannonicum > T. hybridum > T. ambiguum > T. repens > T. montanum (0.092–0.022–0.090 mg g^–1). Regarding the total isoflavone content, the annual clover species were ranked in the sequence: T. incarnatum (3.27 mg g^–1) > T. campestre > T. resupinatum > T. alexandrinum (0.517 mg g^–1).

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