Chemija / Chemistry
 ISSN 0235-7216 ISSN 2424-4538 (online) |
2013 m. Nr. 3 Optimization of matrix solid-phase dispersion extraction
for the chromatographic analysis of flavones in cranberries
A matrix solid-phase dispersion (MSPD) extraction method was applied for three flavones in cranberry (Vaccinium macrocarpon Ait.) matrix for the first time. The optimized MSPD procedure required a small amount of the sample (0.25 g), C18 bonded silica gel (1.0 g) as a dispersant solid-phase sorbent and small volumes of methanol using its aqueous solutions (10 mL) as eluting solvents. Identification and quantification of myricetin, quercetin and kaempferol were performed by HPLC-DAD-MS. The limit of detection varies from 0.04 μg/g to 0.08 μg/g with linear regression coefficients (r2 > 0.999) for each compound. The recoveries of the analytes ranging from 59% to 111% with relative standard deviations less than 9.5% were checked using spiked freezedried cranberry samples. The MSPD method is simple, rapid and efficient for the extraction of flavones from cranberry samples.
Keywords: matrix solid-phase dispersion (MSPD), cranberry, myricetin, quercetin, kaempferol, HPLC-MS |
Issues:
2017 - Vol.28 No. 1, No. 2, No. 3, No. 4 2016 - Vol.27 No. 1, No. 2, No. 3, No. 4 2015 - Vol.26 No. 1, No. 2, No. 3, No. 4 2014 - Vol.25 No. 1, No. 2, No. 3, No. 4 2013 - Vol.24 No. 1, No. 2, No. 3, No. 4 2012 - Vol.23 No. 1, No. 2, No. 3, No. 4 2011 - Vol.22 No. 1, No. 2, No. 3, No. 4 2010 - Vol.21 No. 1, No. 2-4 2009 - Vol.20 No. 1, No. 2, No. 3, No. 4 2008 - Vol.19 No. 1, No. 2, No. 3-4 2007 - Vol.18 No. 1, No. 2, No. 3, No. 4 2006 - Vol.17 No. 1, No. 2-3, No. 4 2005 - Vol.16 No. 1, No. 2, No. 3-4 2004 - Vol.15 No. 1, No. 2, No. 3, No. 4 2003 - Vol.14 No. 1, No. 2, No. 3, No. 4 2002 - Vol.13 No. 1, No. 2, No. 3, No. 4 2001 - Vol.12 No. 1, No. 2, No. 3, No. 4 |