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Chemija / Chemistry

ISSN 0235-7216
ISSN 2424-4538 (online)

2015 m. Nr. 2

Identification of isoflavones and their conjugates in red clover by liquid chromatography coupled with DAD and MS detectors
Lukas TAUJENIS, Audrius PADARAUSKAS, Jovita MIKALIŪNIENĖ, Jurgita CESEVIČIENĖ, Nijolė LEMEŽIENĖ, Bronislava BUTKUTĖ

Ultra performance liquid chromatography (UPLC) coupled with a diode array detector (DAD) and mass spectrometry (MS) was applied for identification of isoflavones and their conjugates in red clover (Trifolium pratense L.) growing in Lithuania. Isoflavones were extracted with methanol/water (9:1, v/v) using sonication for 60 min at room temperature. Optimized separations were carried out on the Acquity UPLC BEH C18 column in the gradient elution mode using the mobile phase composed of water and methanol containing 0.25% (v/v) acetic acid. Based on UV spectra, MS data, elution order and elution profiles, measured before and after acid hydrolysis, a total of 16 isoflavone aglycones, their glucosides and glucoside malonates were identified in red clover extract.
 

Keywords: red clover, isoflavones, conjugates, identification, UPLC-DAD-MS

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