Chemija / Chemistry
 ISSN 0235-7216 ISSN 2424-4538 (online) |
2016 m. Nr. 1 Isolation and characterization of novel pyridine dicarboxylic acid-degrading microorganisms
Five novel microorganisms capable of pyridine dicarboxylic acids degradation were isolated from a soil. Microorganisms utilizing pyridine-2,3-dicarboxylic acid as a sole carbon source were identified as Rhodococcus sp. 23C1, Mycobacterium frederiksbergense 23ON and Cupriavidus campinensis 23K8. This is the first report describing the representatives of these genus capable of degrading this compound. A pyridine-2,3-dicarboxylic acid dehydrogenase (quinolinate dehydrogenase) activity was detected in Rhodococcus sp. 23C1 and Mycobacterium frederiksbergense 23ON. The enzyme was partially purified from Rhodococcus sp. 23C1. Based on detection of nicotinic acid hydroxylase, 6-hydroxynicotinic acid hydroxylase and 2,5-dihydroxypyridine dioxygenase activities in the cell-free extract, a novel pathway of degradation of pyridine-2,3-dicarboxylic acid proceeding via formation of nicotinic acid was proposed for Cupriavidus campinensis 23K8. A bacterial isolate aerobically degrading pyridine-2,6-dicarboxylic acid was identified as Achromobacter sp. JS18. A novel pathway of pyridine-2,6-dicarboxylic acid degradation with 3-hydroxypicolinic acid as an intermediate was proposed for this bacteria. A pyridine-3,5-dicarboxylic acid-degrading bacterial isolate 35KP identified as Xanthobacter sp. was characterized for the first time. A phenazine methosulphate-dependent pyridine-3,5-dicarboxylate dehydrogenase activity was detected in the cell-free extract of Xanthobacter sp. 35KP.
: Keywords: pyridine-2,3-dicarboxylic acid, pyridine-2,6-dicarboxylic acid, pyridine-3,5-dicarboxylic acid, pyridine-3,5-dicarboxylate dehydrogenase, biodegradation |
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