Topsoil and plant material from two plots are investigated. One of the plots was fertilised with wastewater treatment sludge. Normalised intensities (I_n) of Na, Mg, Al, Si, P, S, Cl, K, Ca, Mn, Fe, Ni, Cu, Zn, Rb, Sr, Mo, Cr, Br obtained by EDXRF measuring, with or without a Mylar film, the pressed pellets prepared from plant material ashed at 240 °C using different dilution factors (DF) are compared. Plant material included mainly Festuca rubra and Calamagrostis epigejos. I_n values of Mg, Cr, Ni, Na, Al, P, S, Cl significantly decreased when measuring with the Mylar film, those of Mg, Al, Si, P, S, Cl, K, Ca, Mn, Fe, Zn, Br, Rb, Sr usually significantly decreased with greater dilution of material. It is quite possible to use DF = 0.25 for the preparation of pellets, but it is better to measure without the Mylar film. Despite the influence of plant sample preparation and measurement ways on I_n, the following group of elements with higher than 1.3 times content in the plants of the fertilised plot compared to that of the background plot was distinguished: Zn, Ni, Cu, S, P, Si, Rb, Sr. The contents of these elements, except Si and Rb, also increase in soil of this plot.

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