The characterization of an optical sensor membrane is described for the determination of sulfide ions based on the immobilization of methylene blue on an agarose membrane. Methylene blue is covalently bonded to a transparent epoxy activated agarose film. This optode has a linear range of 6.8 × 10^–6 – 7 × 10^–3 mol L^–1 at 620 nm. The response time of the optode is about 3 min depending on the concentration of sulfide ions. The sensor can readily be regenerated with water, and the color is fully reversible.
The sensor was successfully used to the determination of sulfide in water. The sulfide
optical sensor was mounted in a flow cell and successfully applied for on-line sulfide
monitoring. No evidence of leaching of the dye or any signal drift was observed.

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