 ISSN 0235-7224 ISSN 2029-0586 (online) |
2010 m. Nr. 1-2 Pine defoliator Bupalus piniaria L. (Lepidoptera: Geometridae) and its entomopathogenic fungi. 1. Fungi isolation and testing on larvae
Pine defoliator bordered white moth (pine looper), Bupalus piniaria L. (Lepidoptera: Geometridae) larvae were reared under laboratory conditions and were regularly supplied with pine twigs collected in nature for feeding. Cadavers of naturally infected 2nd and 3rd stage larvae were collected and analysed. Thirty-six fungal isolates belonging to 15 species and 10 genera were obtained, cultivated and identified. Among them two species prevailed: Lecanicillium psalliotae (syn. Verticillium psalliotae) and Fusarium solani, comprising respectively 34.6 and 24.3% of the total number of fungi isolates. Conidial suspensions of the two fungi species at concentrations 105 to 108 conidia/ml were tested. Only the highest concentration of F. solani induced the mortality of 4th instar larvae, although the virulence was low: accumulative mortality ranged from 29.6 to 30.7% after 10 days of spraying. In the control group, the mortality was 15.8% after the same period. The high percentage of cadavers containing one of the two fungal species and the low mortality recorded in the test could be due to the very different sensitivity to the pathogens in different stage larvae. This is the first report on the isolation and identification of fungi developing on B. piniaria. Keywords: pine looper, forest pests, insect pathogens, mortality, microscopic fungi |
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