 ISSN 0235-7224 ISSN 2029-0586 (online) |
2010 m. Nr. 3-4 Bacterial community analysis using temporal temperature gradient gel electrophoresis (TTGE) of 16S rDNA PCR products
of soil metagenome
Separation of amplified 16S rDNA products by temporal temperature gradient gel electrophoresis (TTGE) was carried out for studying bacterial community composition in bulk soil samples in the different tracts (subalpine, temperate and subtropical) of the Western Himalayas (India). Bacterial universal primers were used to amplify the 16S rDNA product. Characteristic profiles of the communities were obtained by TTGE. Bands resolved by TTGE suggested the presence of a moderate diversity among the dominating soil bacterial species of this region. Further, it was observed that the diversity of the bacterial population varies within the same climatic conditions. The study has also revealed that the method is efficient for screening multiple samples. Keywords: TTGE, metagenomics, microbial diversity, the Western Himalayas |
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