Forty-five nasal swab samples were collected from randomly selected calves aged under 3 months without clinical symptoms of pneumonia. Mycoplasmas were isolated from the nasal swab samples of 14 from 45 calves (31.1%).

For confirmation of Mycoplasma bovis species there was used a polymerase chain reaction (PCR) procedure on purified DNA of mycoplasma culture. Primers MBOURC2-L, MBOURC2-R were used to amplify a 1626 bp fragment of Mycoplasma bovis chromosomal DNA. Amplified products were analysed by agarose gel electrophoresis. The expected 1626 bp fragment was obtained in 11 from 14 Mycoplasma bovis field strains tested. According to the obtained data, the PCR method correlated well (78.6%) with cultural and biochemical characteristics of Mycoplasma bovis.

Keywords: Mycoplasma bovis, cultural, biochemical, characteristics, PCR method